Hugh Clarke, PhD

Axe de recherche primaire: 
Human Reproduction and Development
Axe de recherche secondaire: 
Cancer
Courriel: 
Courriel
Le but de la recherche: 

Laboratory studies focus on mammalian oogenesis and early embryogenesis. Three projects are being pursued; 1) The role of an RNA-binding protein, termed stem-loop binding protein (SLBP), in regulating the accumulation and translation of mRNAs encoding histones in oocytes and embryos, and the mechanisms that control the synthesis and degradation of SLBP in these cellsl; 2) The process and mechanisms that assemble the paternal DNA into functional chromatin at fertilization; 3) The mechanism by which at a specific stage of its growth the oocyte acquires the ability to undergo the terminal phase of oogenesis, which is a transition from G2 to M-phase of the cell cycle.Reproduction, oogenesis, fertilization, chromatin, histones, cell cycle, translational regulation

Mots-clés: 
Reproduction, oogenesis, fertilization, chromatin, histones, cell cycle, translational regulation
Emplacement: 
Royal Victoria Hospital
Publications:
Bedard, N., Y. Yang, M. Gregory, D. G. Cyr, J. Suzuki, X. Yu, R.-C. Chian, L. Hermo, C. O’Flaherty, C. E. Smith, H. J. Clarke and S. S. Wing. Mice lacking the USP2 deubiquitinating enzyme have severe male sub-fertility associated with defects in fertilization and sperm motility. Biol. Reprod. 85, 594-604; 2011
Elizur, S.E., W. Y. Son, H. Clarke, D. Morris, Y. Gidoni, E. Demirtas and S. L. Tan. A unique biological in-vivo model to evaluate follicular development during in-vitro maturation treatment. Reprod. Biomed. Online 22, 257-262; 2011
Yang, Q., P. Allard, M. Huang, W.L. Zhang, and H.J. Clarke. Proteasomal activity is required to initiate and to sustain translational activation of mRNA encoding the stem-loop-binding protein during meiotic maturation. Biol. Reprod. 82, 123-131; 2010
Zhang, W.L., L. Poirier, M.M. Diaz, V. Bordignon and H.J. Clarke. Maternally encoded stem-loop-binding protein is degraded in 2-cell mouse embryos by the co-ordinated activity of two separately regulated pathways. Dev. Biol. 328, 140-147; 2009
Arnold, D.R., P. Françon, J. Zhang, K. Martin and H.J. Clarke. Stem–loop binding protein expressed in growing oocytes is required for accumulation of mRNAs encoding histones H3 and H4 and for early embryonic development in the mouse. Dev. Biol. 313, 347-358; 2008
Mohamed, O.A., M. Jonnaert, C. Labelle-Dumais, K. Kuroda, H. J.Clarke and D. Dufort. Uterine Wnt/ â-catenin signaling is required for implantation. Proc. Nat. Acad. Sci. 102, 8579-8584; 2005